Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.

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961 – 975 of 5,382 results

961

Thermo Scientific™ CDK7/9tide Substrate

A peptide substrate based upon the C-terminal tail of RNA Polymerase II that is a reported substrate for Cyclin Dependent Kinases (CDK7, CDK8 and CDK9).

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962

Kinase Buffer S (5X)

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963

Promega Ribonuclease H

RNase H is an endonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA.

Pricing & Availability
Specifications

Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	Ribonuclease H
Storage Buffer	20mM HEPES-KOH (pH 7.8 at 25°C), 1mM DTT, 50mM KCl, 0.2mg/mL BSA, 50% Glycerol
Color	Colorless
pH	7.8
Concentration	0.5 to 2 U/μL
For Use With (Application)	Removal of the RNA strand prior to second strand cDNA synthesis, analysis of in-vitro polyadenylation reaction products, microarrays
Source	Recombinant E. coli strain

964

Promega T4 DNA Ligase

For ligation of DNA ends.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 300mM Tris HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP
Content And Storage	-30°C to -10°C
Form	Liquid
Product Type	T4 DNA Ligase
Storage Buffer	10mM Tris HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 50% Glycerol
Molecular Weight (g/mol)	68
Color	Colorless
pH	7.4
Purity	>90%
For Use With (Application)	Joining double-stranded DNA molecules with cohesive or blunt ends (5', 3' or blunt-ended DNA inserts)
Source	Recombinant E. coli strain

965

Promega T4 Polynucleotide Kinase

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

Pricing & Availability
Specifications

Components	10X Reaction Buffer: 700mM Tris HCl (pH 7.6 at 25°C), 100mM MgCl2, 50mM DTT
Content And Storage	-30°C to -10°C
Inhibitors	Ammonium Ions and low levels of Phosphate Buffers
Form	Liquid
Product Type	T4 Polynucleotide Kinase
Storage Buffer	20mM Tris HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP, 50% Glycerol
Molecular Weight (g/mol)	132
Color	Colorless
pH	7.5
Purity	≥90%
Concentration	5 to 10 U/μL
For Use With (Application)	5′ End-labeling of single or double-stranded DNA and RNA molecules for use as probes, sequencing or DNA Protein footprinting, Phosphorylation of DNA prior to cloning
Source	Recombinant E. coli strain